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video_tutorials/protein_binder_design/README.md

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+# Files for the Protein Binder Design with RFdiffusion Video Tutorial
+
+The files in this directory include example outputs and analysis scripts to go along with a video tutorial for how to use RFdiffusion to design protein binders. You can find the corresponding video tutorial on the [Rosetta Commons YouTube channel](https://www.youtube.com/@RosettaCommons).
+
+**Part1-Diffusion** contains a shell script you that runs RFdiffusion to create the protein binders shown in the video. The various configuration options in this file are discussed in the video, but you can also take a look at the [RFdiffusion documentation](https://sites.google.com/omsf.io/rfdiffusion/reference-docs/configuration-options?authuser=0) for more information. 
+
+Also included: 
+- `channel-toxin.pdb`: the trimmed and cleaned input PDB file.
+- `outputs-test`: a directory containing 10 example output PDB and TRB files.
+
+**Part2-Backbone-assessment** contains two analysis scripts (`analyze_backbones.py` and `filter_backbones.py`) that are used in the video to discern which designs actually fit the necessary design parameters. You can run 
+
+Also included: 
+- `metrics.csv`: the data generated by `analyze_backbones.py` for the structures created in Part 1 of the tutorial.
+- `outputs-test`: a directory containing 10 example output PDB and TRB files.
+
+## Authors
+The materials in this directory were created by Diego Lopez Mateos, Matthew Hvasta, and Kush Narang for the Tutorial Hackathon track of the 2026 Megathon event hosted by Rosetta Commons. The video tutorial was edited by Austin Seamann.